MBF Labs scientists and technicians collect theĭata, interpret the results, and prepare a final report. Labs services are ideal for anyone looking forĮxpert stereological analysis but lacking in-house instrumentation orĮxpertise. Ongoing work in numerous fields, and it has contributed to recentĪdvances in disease research, including Alzheimer’s,Ĭomponent of stereological analyses for pharmaceutical, biomedical, and Stereo Investigator is an integral component of Peer-reviewed journal articles-more than any other stereology
Mapping, and measurement all in one software package. Investigator is the only complete, scalable solution fromīrightfield to confocal stereology, with tools for image analysis, The field of stereology, the emphasis is on the use of stereology inīiological research, though anyone interested in learning the basicĬoncepts of stereology will find something of interest. Designed to introduce both basic and advanced concepts in Provides the most comprehensive information about stereology The central nervous system, as well as other organs, has contributedĬonsiderably to our understanding of the functional and pathological TheĪpplication of design-based stereological methods to the analysis of Recent years, advent of design-based stereology has improved theĪccuracy and reliability of quantification in biological research. Length of capillaries, the volume of a tumor, as well as the percentage Examples of thisĪre the number of brain cells within a brain region, the In fact, today the most commonĮstimates are quantities in 3-dimensional material. The field of stereology has evolved to include the study
The more challenging concepts deal with theĮstimation of 3-dimensional quantities based on the observation of Inquiries are invited concerning membership,Īre many examples today of estimating geometric quantities thatĪre 2-dimensional. Microanatomy, cytology, ultrastructure studies, metallurgy, geology,Īstronomy, and cosmology. The exchange of ideas concerning applicable research methods in Three-dimensional interpretation of flat images) has been formed for
"An International Society of Stereology (the science of
Information from flat, two-dimensional images.ġ961, the journal Science carried the following The main disadvantage of IF, however, is that it necessarily destroys the tissue analyzed and thus provides no spatial information on the cellular composition of biological regions of interest.Is simply the study of estimating geometric Tissue shrinkage is also not an issue, since the estimates provided are independent of tissue volume. IF is as accurate as unbiased stereology and faster than stereological techniques, as it requires no elaborate histological processing or sampling paradigms, providing reliable estimates in a few days rather than many weeks. We compare the advantages and disadvantages of each method and provide researchers with guidelines for choosing the best method for their particular needs. This method entails transforming a highly anisotropic tissue into a homogeneous suspension of free-floating nuclei that can then be counted under the microscope or by flow cytometry and identified morphologically and immunocytochemically as neuronal or non-neuronal. An alternative, the isotropic fractionator (IF), became available in 2005 as a fast and inexpensive method that requires little training, no specific software and only a few materials before it can be used to quantify total numbers of neuronal and non-neuronal cells in a whole organ such as the brain or any dissectible regions thereof. Obtaining unbiased estimates of cell numbers, however, was until recently possible only through stereological techniques, which require specific training, equipment, histological processing and appropriate sampling strategies applied to structures with a homogeneous distribution of cell bodies. The number of cells comprising biological structures represents fundamental information in basic anatomy, development, aging, drug tests, pathology and genetic manipulations.